Attia, A., Elmetwalli, A. (2021). Screening of Occult Hepatitis B Virus Infection among Egyptian Blood Donors.. Medical Journal of Viral Hepatitis, 5.2(2), 27-31. doi: 10.21608/mjvh.2021.158276
Attia Ahmed Attia; Alaa Elmetwalli. "Screening of Occult Hepatitis B Virus Infection among Egyptian Blood Donors.". Medical Journal of Viral Hepatitis, 5.2, 2, 2021, 27-31. doi: 10.21608/mjvh.2021.158276
Attia, A., Elmetwalli, A. (2021). 'Screening of Occult Hepatitis B Virus Infection among Egyptian Blood Donors.', Medical Journal of Viral Hepatitis, 5.2(2), pp. 27-31. doi: 10.21608/mjvh.2021.158276
Attia, A., Elmetwalli, A. Screening of Occult Hepatitis B Virus Infection among Egyptian Blood Donors.. Medical Journal of Viral Hepatitis, 2021; 5.2(2): 27-31. doi: 10.21608/mjvh.2021.158276
Screening of Occult Hepatitis B Virus Infection among Egyptian Blood Donors.
1Department of Microbiology, Faculty of Science, Benha University, Benha, Egypt.
2Department of Clinical Trial Research Unit, Egyptian Liver Research Institute and Hospital, Mansoura, Egypt.
Abstract
Background:Although sensitive screening assays for hepatitis B virus surface antigen (HBsAg) are available, special cases of post-transfusion hepatitis B virus infection still occur. The present study was conducted to evaluate the prevalence of anti-hepatitis B core (anti-HBc) positivity and the presence of HBV-DNA in serum samples of healthy blood donors negative for both HBsAg and anti-HCV antibodies in Benha, Egypt. Materials and methods: The study included a screening of 450 selected blood donors. The distribution of blood donors was 288 males (64%) and 162 females (36%). The recruited blood donors who met the criteria for blood donation were routinely screened for HBsAg, HIV I/2-Ab, and syphilis-antibodies. The blood units for donations were further analyzed for the presence of HBc-IgM and HBV-DNA levels by PCR method. Results: Testing of the accepted units for the donation was about 12 (2.7%) HBc-IgM positive, and 9 (2%) HBV-DNA positive units. The standard screening of blood unit failed to recognize early acute or window HBV infection where HBsAg is missing. Conclusions:Our investigations proposed that sensitive methods for the detection of HBV by PCR might be recommended in the screening of donated blood. Furthermore, anti-HBc antibodies should be tested regularly on all blood donation units.
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