Egyptian Liver Research Institute and Hospital (ELRISH)Medical Journal of Viral Hepatitis2314-87481.2220160601Interferon-ë rs12979860CC genotype predicts sustained virological response to therapy in patients with chronic hepatitis C Genotype 4 but enhances hepatic fibrosis112456610.21608/mjvh.2016.4566ENShiha G.Internal Medicine dept., Faculty of Medicine, Mansoura Univ., Egypt.
,Egyptian Liver Research Institute and Hospital (ELRIAH), Mansoura, Egypt.Samir W.Egyptian Liver Research Institute and Hospital (ELRIAH), Mansoura, Egypt.
,Biochemistry dept., Faculty of Medicine, Mansoura Univ., Mansoura, EgyptSeif S.Internal Medicine dept., Faculty of Medicine, Mansoura Univ., Egypt.
,Egyptian Liver Research Institute and Hospital (ELRIAH), Mansoura, Egypt.Soliman R.Egyptian Liver Research Institute and Hospital (ELRIAH), Mansoura, Egypt.Zalata K.Cairo, Pathology dept., Faculty of Medicine, Mansoura Univ., Egypt.Helmy A.Tropical Medicine & Gastroenterology, dept., Faculty of Medicine, Assiut Univ., Assiut, Egypt.Journal Article20160411Recently, several genome wide association studies (GWAS) have revealed that several single nucleotide polymorphisms (SNPs) in proximity to the interleukin (IL)-28B genes can predict spontaneous clearance of hepatitis C virus (HCV)<br />infection as well as sustained virological response (SVR) to Pegylated interferon-2a/b plus ribavirin therapy. In HCV-genotype 1 patients, the allele rs12979860 is associated with greater hepatic necro-inflammation and worse clinical outcome, but lower fibrosis progression. Data on patients infected with HCV genotype 4 (G4) is limited. The present study assesses the impact of rs12979860 IL28 SNP on hepatic fibrosis and SVR in patients with chronic HCV G4 infection. A total of 163 patients with chronic HCV G4 infection (123 males and 40 females) who had pre-treatment liver biopsy were<br />included. Both HCV RNA and IL28 rs12979860 genotypes were determined by real time polymerase chain reaction. Hepatic fibrosis, IL28B rs12979860 genotype, and SVR were statistically correlated together and with various<br />clinical, biochemical, and virological parameters. All patients were found to be infected with HCV G4. IL28B rs12979860 genotypes TT, CT, and CC were detected in 29 (17.8%), 107 (56.6%), and 27 (16.6%) patients, respectively.<br />SVR was achieved in 85 (52.1%), severe fibrosis (≥F3) by METAVIR score was found in 75 (64.0%). The CC allele is associated with nearly twice (OR=1.898) the risk for having severe fibrosis (≥F3), but nearly 16 times SVR (OR=15.833) than having CT and TT. Contrary to HCV G1, the CC allele at IL28B rs12979860 appears to be associated with higher hepatic fibrosis, but better SVR to therapy in patients with chronic HCV G4 infection. Egyptian Liver Research Institute and Hospital (ELRISH)Medical Journal of Viral Hepatitis2314-87481.2220160601A simple bedside blood test (Fibrofast; FIB-5) is superior to FIB-4 index for the differentiation between non-severe and severe fibrosis in patients with chronic Hepatitis C110456710.21608/mjvh.2016.4567ENShiha G.Gastrohepatology Unit, Internal Medicine dept., Faculty of Medicine, Mansoura Univ.,
Egypt.Samir W.Egyptian Liver Research Institute and Hospital (ELRIAH), Mansoura, EgyptSeif S.Gastrohepatology Unit, Internal Medicine dept., Faculty of Medicine, Mansoura Univ.,
Egypt.Eldesoky AGastrohepatology Unit, Internal Medicine dept., Faculty of Medicine, Mansoura Univ.,
Egypt.Elbasiony M.Gastrohepatology Unit, Internal Medicine dept., Faculty of Medicine, Mansoura Univ.,
Egypt.Soliman R.Egyptian Liver Research Institute and Hospital (ELRIAH), Mansoura, Egypt.Metwally A.Community Medicine Department, National Research Center, Cairo, EgyptZalata K.Pathology Department, Faculty of Medicine, Mansoura University, Egypt.Journal Article20160302A simple noninvasive score (Fibrofast, FIB-5) was developed using five routine laboratory tests (ALT, AST, Alkaline phosphatase, Albumin and Platelets count) for the detection of severe hepatic fibrosis in patients with chronic hepatitis C. The FIB-4 index is a noninvasive test for the assessment of liver fibrosis, and a score of ≤3.35 enables the correct identification of patients who have nonsevere (F0-2) from severe fibrosis (F3 4), and could avoid liver biopsy. The aim of this study was to compare the performance characteristics of FIB-5 and FIB-4 to differentiate between nonsevere<br />from severe fibrosis. A cross-sectional study included 604 chronic HCV patients. All liver biopsies were scored using<br />METAVIR system. Both FIB-5 and FIB-4 scores were measured and the performance characteristics were calculated using the ROC curve. The performance characteristics of Fibro-Fast at ≥ - 2.1 and FIB-4 at ≤ 3.25 for the differentiation between non-severe fibrosis and severe fibrosis were; sensitivity 39.6%, NPV 88.7% and sensitivity 29.7%, NPV 87.4% respectively. Conclusion: FIB-5 score at the new cutoff is more superior to FIB-4 index for the differentiation between non- severe and severe fibrosis. Egyptian Liver Research Institute and Hospital (ELRISH)Medical Journal of Viral Hepatitis2314-87481.2220160601Transient elastography (FibroScan) is not useful in the diagnosis of schistosomal hepatic fibrosis110456810.21608/mjvh.2016.4568ENShiha G.Gastrohepatology Unit, Internal Medicine dept., Faculty of Medicine, Mansoura Univ., Egypt.Samir W.Egyptian Liver Research Institute and Hospital (ELRIAH), Mansoura, Egypt.Soliman R.Egyptian Liver Research Institute and Hospital (ELRIAH), Mansoura, Egypt.Elbasiony MGastrohepatology Unit, Internal Medicine dept., Faculty of Medicine, Mansoura Univ., Egypt., Egyptian Liver Research Institute and Hospital (ELRIAH), Mansoura, Egypt.Ahmed NEgyptian Liver Research Institute and Hospital (ELRIAH), Mansoura, Egypt.Helmy A.Egyptian Liver Research Institute and Hospital (ELRIAH), Mansoura, Egypt.
,Tropical Medicine and Gastroenterology dept., Faculty of Medicine, Mansoura Univ.,Journal Article20160323Transient Elastography (TE) is a widely-used noninvasive measure of liver stiffness. This study aimed to evaluate the diagnostic accuracy of TE in the diagnosis of schistosomal hepatic fibrosis (SHF). A total of 30 patients (Mean±SD age 42.1±8.8 years) with pure schistosomiasis were included. Abdominal ultrasound (US) and upper gastrointestinal endoscopy were performed to all patients to assess for signs of portal hypertension and the presence of varices as sequels of SHF. TE (FibroScan) was done to determine liver stiffness. A cutoff value of ≥10.1 Kpa indicates advanced fibrosis (F3-4). Splenomegaly was detected in 27(90 %) patients and was moderate to marked (≥15cm) in 19 (63.3 %). Esophageal and gastric varices were found in 25 (83.3 %) and 4 (13.3 %) cases respectively. TE was successful in all patients, and the mean ± SD liver stiffness was 9.4 ± 5.5 Kpa (Range: 3.5-30 Kpa).F3-4 by FibroScan was detected in 8/25 (32.0 %) and 9/27 (33.3 %) of patients with EV and splenomegaly respectively. Similarly, F3-4 was absent in 4/5(80.0 %) and3/3 (100 %) of those without EV and splenomegaly advanced fibrosis respectively (p=0.71 and p=0.27 respectively). To our knowledge, this study shows for the first time that TE is not useful in diagnosis of SHF and EV in patients with pure schistosomiasis. Whether this is applicable to other cases of prehepatic portal hypertension, such as portal vein thrombosis and congenital hepatic fibrosis, needs further investigation.